System Suitability in HPLC Analysis
by HPLC chromatographic technique is used in pharmaceutical industries for analysis. System suitability testing limits are acceptance criteria that must met before starting the analysis. There …
MACO and NOEL Calculation for Cleaning Validation
MACO and NOEL calculations have great importance in the pharmaceutical industries. NOEL refers to “No observed effect level” and MACO is a “Maximum allowable carryover”. …
UV-Visible Spectroscopy
Ultraviolet-visible (UV-Vis) spectroscopy is a widely used technique in many areas of science ranging from bacterial culturing, drug identification and nucleic acid purity checks and quantitation, …
Detectors for Chromatography
What are detectors in chromatography A chromatography detector is a device used in GC or LC to detect components of the mixture being eluted off the chromatography column. There …
Gas chromatography
Gas chromatography (GC) is an analytical technique used to separate and detect the chemical components of a sample mixture to determine their presence or absence and/or …
Cleaning Validation
What is cleaning Validation Cleaning validation is the methodology used to assure that a cleaning process removes chemical and microbial residues of the active, inactive …
Forced Degradation
The FDA and ICH the requirement of stability testing data to understand how the quality of a drug substance and drug product changes with time …
ELSD Detector
An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC), Ultra high-performance liquid chromatography (UHPLC), Purification liquid chromatography such as …
RI Detector
RI detectors monitor the difference in RI between the column eluent containing analyte and a reference stream containing mobile phase only. These detectors are the closest …
PDA Detector
What is PDA detector Photodiode array detectors—variously abbreviated as “PDA detectors” or simply “DADs”—are essentially spectrophotometers that transiently measure the absorbance of light by a liquid …