by 1.0 OBJECTIVE:
To lay down a procedure for operation & cleaning of horizontal autoclave.
2.0 SCOPE:
This procedure is applicable for operation & cleaning of horizontal autoclave.
3.0 RESPONSIBILITY:
Microbiologist
4.0 ACCOUNTABILITY:
QC Manager.
5.0 PROCEDURE:
5.1 Operation Procedure
- Ensure the instrument free form dust.
- Connect the power plug.
- Check the water level before operate the instrument.
- Fill the article for sterilization and close the door of autoclave carefully and tightly.
- ON the instrument.
- Set the required temperature by select arrow mark up and down arrow keys.
- Set the required holding time by select arrow mark up and down arrow keys.
- Press the start key for sterilization of materials.
- After completion of sterilization, horn the buzzer.
- Press the ENT key for OFF the sterilization.
- OFF the main instrument.
- Record maintained of usage of autoclave .
5.2 Cleaning for Vertical Autoclave:-
- Ensure the power supply is OFF before starting cleaning operation, Disconnect the main power plug.
- De dust the autoclave from outside.
- Open the cover from and drain the water from autoclave.
- Outside and inner side wash by water and then wop with dry cloth and then with wop with 70% IPA.
- Record the details in autoclave cleaning record .
- Frequency:- Daily cleaning after use of instrument.
6.0 TRAINING:
Trainer : Manager – Quality Control
Trainees : Staff of the microbiology departments
7.0 DISTRIBUTION:
Master Copy : QA Department
Controlled Copy : Microbiology Department
Display Copy : Microbiology Department (If Required)
8.0 ATTACHMENTS:
| Sr. No. | Title |
| 01. | Usage log book of Autoclave |
| 02. | Cleaning Record of Autoclave |
9.0 REFERENCES:
In-House
10.0 ABBREVIATIONS:
| Abbreviations | Extended Form |
| SOP | Standard Operating Procedure |
| NA | Not Applicable |
| MB | Microbiology |
| Sr. No. | Serial Number |
| QA | Quality Assurance |
| DEPT. | Department |
| IPA | Isopropyl Alcohol |
| % | Percentage |
11.0 REVISION HISTORY OF CHANGE:
| Sr. No. | Date | Revision Details | Revision No. |
| 01 | …….. | NEW SOP | 00 |
