WATER SAMPLING FOR MICROBIOLOGICAL ANALYSIS

1. OBJECTIVE:

This SOP Describes the Procedure for Water Sampling for Microbiological Analysis.

• SCOPE:

This SOP is Applicable to the Procedure for Water Sampling for Microbiological Analysis.

• RESPONSIBILITY:

         Microbiologist

• ACCOUNTABILITY:

Head Quality Control.

• PROCEDURE
  • Sampling Procedure:-
  • Sampling should be done by trained microbiologist.
  • Sterilize the bottles in which the sample is to be collected.
  • Add 2ml of 3% Sodium Thiosulphate into the sampling bottles and then sterilize the bottles for samples containing residual chlorine.
  • Carry the sterilized bottles to the sampling point in SS sampling box sanitized with 70%
    IPA.
  • Before starting the sampling wear hand gloves and face mask.
  • Sanitize the hands with 70% IPA.
  • Flush water through sampling point for 5 – 20 Seconds. Where ever there is no drain carry the
    bucket to collect the water while flushing and avoid spillage of water on the floor.
  • Collect the 100ml of water sample for self analysis and 500ml of water for outside lab for microbiological analysis into sterilized bottles.
  • Keep the bottles back in the SS sampling box and carry it to the microbiology lab.
  • If the bottle with sample breaks, while transit or while performing the analysis invalidate
    the test, document the same and freshly collect the sample on the same day in other sterilized bottle.
  • If for any reason, the sample is not tested immediately, store it in the refrigerator 2 – 8°C
    for not more than 4 hrs.
  • If the sample is stored in the refrigerator for not more than 4 hrs, invalidate the test and
    document the same.
  • If sample send out side for microbiological analysis in ice box / cool pad and should be maintain the 2 – 8⁰C temperature. If the temperature in not maintain sample is invalid for same analysis.
  • Outside sample should be analysis within 24 hour form time of sampling.
• TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the microbiology departments

• DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           Microbiology Department

Display Copy                 :           Microbiology Department (If Required)

• ATTACHMENTS:

Sr. No.	Title	Annexure No.
01.	NA	NA

• REFERENCES:

In-House

1. ABBREVIATIONS:

Abbreviations	Extended Form
SOP	Standard Operating Procedure
NA	Not Applicable
MB	Microbiology
Sr. No.	Serial Number
QA	Quality Assurance
DEPT.	Department
IPA	Isopropyl Alcohol
%	Percentage

1. REVISION HISTORY OF CHANGE:

Sr. No.	Date	Revision Details	Revision No.
01	NA	NEW SOP	NA
02	NA	SOP Periodical  Review	NA