PROCEDURE FOR OPERATING PROCEDURE FOR ANTIBIOTIC ZONE READER

1. OBJECTIVE:

To lay down a procedure for operating procedure for antibiotic zone reader.

• SCOPE:

This procedure is applicable for procedure for operating procedure for antibiotic zone reader.

• RESPONSIBILITY:

Microbiologist

• ACCOUNTABILITY:

QC Manager.

•  PROCEDURE:
  • Check that the instrument is clean and kept in proper position.
  • Connect the power cable to the mains and switch ‘ON’.
  • Switch ‘ON’ the mains in the instrument.
  • Ensure that the zero of the main scale coincides with zero of the main scale coincides with zero in the vernier scale.
  • Place the petri dish in such a way that the lower edge of the zone coincides with the mark line on the prism and in the centre of the magnifier.
  • Rotate the left side knob anticlockwise till the upper edge of the zone coincides with the mark line on the prism.
  • Note down the reading in mm with respect to the main scale and vernier.
  • Rotate the stage anticlockwise using the handle provided at the left hand side and bring it to the zero position.
  • After completion of work switch ‘OFF’ the mains.
  • Maintain the usage record of antibiotic zone reader.   
• TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the microbiology departments

• DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           Microbiology Department

Display Copy                 :           Microbiology Department (If Required)

• REFERENCES:

In-House

1. ABBREVIATIONS:

Abbreviations	Extended Form
SOP	Standard Operating Procedure
NA	Not Applicable
MB	Microbiology
Sr. No.	Serial Number
QA	Quality Assurance
DEPT.	Department
IPA	Isopropyl Alcohol
%	Percentage