PROCEDURE FOR OPERATION & CALIBRATION OF WATER BATH

1.0 OBJECTIVE:

To lay down a procedure for Operation & Calibration of water bath.

2.0 SCOPE:

This procedure is applicable for procedure for Operation & Calibration of water bath .

3.0 RESPONSIBILITY:

Microbiologist  

4.0 ACCOUNTABILITY:

QC Manager.

 5.0 PROCEDURE:

5.1 Operating Procedure:

• Ensure that the instrument is clean and suitable for use.
• Ensure that all the switches are ‘OFF’ in normal position and the level of water in the water bath is up to the mark.
• Switch ‘ON’ the main power supply and then switch ‘ON’ the button of the instrument.
• Set the desired temperature of water bath by press the both set & up or down arrow key.
• Desired temperature shown in red colour & set temperature shown in green colour. 
• After heating the water, start your work carefully.
• After completion the work, switch ‘OFF’ the Instrument.
• Maintain its usage log book .

5.2 Calibration:

• Start the instrument and set the temperature at 50⁰C.
• After achieved the 50⁰C temperature; check the temperature by calibrated thermometer and again check the temperature after 10 minutes.
• 5.2.1 to 5.2.2 parameter follow for 60⁰C, 70⁰C, 80⁰C, 90⁰C & 100⁰C one by one.
• Acceptance Criteria is ±2.0⁰C of set temperature.
• Maintain the calibration record of water bath .
• Frequency of the calibration – Monthly.

5.3 Precautions:

• Instrument should be handled properly.
• Do not dip the hand in water during on the instruments.
• If the instrument does not produce satisfactory results it should be labeled ‘Under Maintenance’ or ‘Out of Order’.

6.0 TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the microbiology departments

7.0 DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           Microbiology Department

Display Copy                 :           Microbiology Department (If Required)

8.0 ATTACHMENTS:

Sr. No.	Title
01.	Usage Log Book of Water Bath
02.	Calibration Record of Water Bath

9.0 REFERENCES:

In-House

10.0 ABBREVIATIONS:

Abbreviations	Extended Form
SOP	Standard Operating Procedure
NA	Not Applicable
MB	Microbiology
Sr. No.	Serial Number
QA	Quality Assurance
DEPT.	Department

11.0 REVISION HISTORY OF CHANGE:

Sr. No.	Date	Revision Details	Revision No.
01	…………	NEW SOP	00