CALIBRATION RECORD OF TLC CABINET

PROCEDURE

For 254 nm (For Short Wavelength):

Prepare a 0.04 % w/v solution of Sodium Salicylate in ethanol (95%).

• Weight…………………..(40 mg or 0.040 gm) of Sodium Salicylate and dilute to 100 ml of ethanol (95%).
• Apply 5µl of this solution on silica gel G with a diameter of about 5mm and examine visually.
• The distance between the lamp and the plate under examination used in Pharmacopeial test should not exceed the distance used to carry out the above test.
• The lamp should be capable of revealing without doubt a standard spot of Sodium Salicylate.

For 365 nm (For Long Wavelength):

Prepare a 0.2 % w/v solution of Sodium Salicylate in ethanol (95%).

• Weight…………………..(200 mg or 0.20 gm) of Sodium Salicylate and dilute to 100 ml of ethanol (95%).
• Apply 5µl of this solution on silica gel G with a diameter of about 5mm and examine visually.
• The distance between the lamp and the plate under examination used in Pharmacopeial test should not exceed the distance used to carry out the above test.
• The lamp should be capable of revealing without doubt a standard spot of Sodium Salicylate.

Result: Pass /Fail

Calibration Status                  :

Corrective action (If any)      :