by Procedure:
Weighed accurately _________________gm (50.0g) of Sucrose which is previously dried at 105°C for 3 hours into a ___________ml (100 ml) volumetric flask. Dissolved and diluted to volume with purified water. (Solution A) [50 % Solution].
| Diluted ___________ ml (5 ml) of Solution A to _______ ml (25 ml) with purified water (Solution B) [10% Solution] |
| Diluted ___________ ml (10 ml) of Solution A to _______ ml (25 ml) with purified water (Solution B) [20% Solution] |
| Diluted ___________ ml (30 ml) of Solution A to _______ ml (50 ml) with purified water (Solution B) [30% Solution] |
| Diluted ___________ ml (20 ml) of Solution A to _______ ml (25 ml) with purified water (Solution B) [40% Solution] |
| Concentration of Sucrose (gm/100ml) | Angle of Rotation (+) at 250 | Observed of Optical Rotation | Variation |
| 10.0 | 13.330 | ||
| 20.0 | 26.610 | ||
| 30.0 | 39.860 | ||
| 40.0 | 53.060 | ||
| 50.0 | 66.230 |
Result: Pass /Fail
Calibration Status :
Corrective action (If any) :
