Standard Operating Procedure for Calibration of Laboratory Glassware

1. OBJECTIVE:

To lay down a procedure for calibration of laboratory glassware.

• SCOPE:

This procedure is applicable for procedure for calibration of laboratory glassware.

• RESPONSIBILITY:

QC Officer/ QC Executive

• ACCOUNTABILITY:

QC Manager.

•  PROCEDURE:
  • General Instructions:
    • Rinse the glassware with potable water.
    • Soak the glassware in Teepol / detergent solution /Sulphuric acid.
    • Remove the glassware and clean with proper brushing.
    • Properly rinse the glassware with tap water and then with distilled or purified water.
    • Finally rinse with distilled or purified water.
    • Allow the glassware to drain completely.
    • Dry the glassware in hot air oven with adequate care.
  • Calibration:
    • Remove glassware from the oven and allow cooling to room temperature.
    • In the room where the balance kept, ensure that the temperature must be 27± 2 ⁰C.
    • Arrange a water bath consisting of a plastic beaker containing water at 27 ºC.
    • Place the glass ware to be calibrated on the single pan balance & press the tare switch.
    • Remove the glass ware and transfer the distilled water at 27ºC so that the meniscus of liquid.
    • Weigh the glass ware and note down weight of water up to 4 decimal places similarly transfer water at 25ºC so that weight.
    • Multiple each weight of successive 1 ml graduation with wt. /ml of water at 27ºc i.e.0.99602 g to obtained actual volume in ml.
    • For Volumetric Flask Grade A:

Nominal capacity ( ml )	Tolerance
10	± 0.025 ml
25	± 0.04 ml
50	± 0.06 ml
100	± 0.10 ml
200	± 0.15 ml
250	± 0.20 ml
500	± 0.25 ml
1000	± 0.50 ml

• For One Mark Pipettes Grade A:

Nominal capacity ( ml )	Tolerance
1	± 0.01 ml
2	± 0.01 ml
5	± 0.01 ml
10	± 0.02ml
20	± 0.03 ml
25	± 0.05 ml

• For Graduated Pipettes Grade A:

Nominal capacity ( ml )	Tolerance for 1 ml subdivisions
1	± 0.006 ml
2	± 0.01 ml
5	± 0.03 ml
10	± 0.05 ml
25	± 0.10ml

• For Burettes Grade A:

Nominal capacity ( ml )	Tolerance
25	± 0.05 ml
50	± 0.05 ml

• For Measuring Cylinder Grade A:

Nominal capacity ( ml )	Tolerance
10	± 0.25 ml
25	± 0.25 ml
50	± 0.5 ml
100	± 0.5 ml
250	± 1.0 ml
500	± 1.0 ml
1000	± 1.0 ml

• Precaution Take adequate care while handling acids and corrosive chemicals.Ensure thorough cleaning or glassware so that previous residues are totally removed.
• Glass wares Handle with care.Switch ‘OFF’ the oven during glass ware handling.
• TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the QC departments

• DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           QC Department

Display Copy                 :           QC Department

• REFERENCES:

In-House