SOP for Calibration of Gas Chromatography

1. OBJECTIVE:

To lay down a procedure for operation and calibration of Gas Chromatography.

• SCOPE:

This procedure is applicable for procedure for operation and calibration of Gas Chromatography.

• RESPONSIBILITY:

QC Officer/ QC Executive

• ACCOUNTABILITY:

QC Manager.

• PROCEDURE:
• Precautions:Do not heat the G.C. column without passing carrier gas through the column.
• Check the leakage of Hydrogen gas before putting the flame on.Check frequently the blue Silica gel of Gas filtration unit and  replace before it turns completely white.
• Ensure that no air bubble is entrapped in syringe while injecting the sample.
• If found any difficulty in operating the instrument or G.C. Solution software, and then refer the instrument manual.
• Inform to the Department Head, If any abnormality is observed while working with Gas chromatograph
•    Column Oven Temperature Calibration:  

               Ware google and gloves while handling glass wool.

                         Insert the calibrated thermometer from top side by piercing glass wool.

                         Set column oven temperature on GC to 50⁰C, 100⁰C, 200⁰C, and 250⁰C.

                Allow to stabilize the set temperature for 5 min and record temperature on the

                       Digital thermometer and respective temperature.                                                                                        

• Detector Calibration:
• Operate the instrument as per its standard operating procedure.
• Prepared the solution as follows:Prepare 1.0% v/v toluene in n-Hexane.
• Follow the GC parameters as given:

Oven Temp.: 110⁰C(isothermal)

Injector Temp.: 180⁰C

Column Flow: 2.7 ml/min.

Run Time: 5 minutes.

Injector Volume: 0.5µl.

• Inject the 0.5 ml of test preparation and record the chromatograph.
  • Inject blank and then six injections of 1.0% toluene solution in system and record chromatogram for all injections.
    • Calculate the RSD for the area of six 1.0% toluene injections.
    • The RSD should not be more then 15.0%.
    • Frequency of calibration is in every three month.
• TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the QC departments

• DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           QC Department

Display Copy                 :           QC Department (If Required)

• ATTACHMENTS:

Sr. No.	Title	Annexure No.
01.	NA	NA

• REFERENCES:

In-House

1. ABBREVIATIONS:

Abbreviations	Extended Form
SOP	Standard Operating Procedure
NA	Not Applicable
QC	Quality Control
Sr. No.	Serial Number
QA	Quality Assurance
DEPT.	Department

1. REVISION HISTORY OF CHANGE:

Sr. No.	Date	Revision Details	Revision No.

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