Calibration of Volumetric Apparatus

1. Objective:

To lay down a procedure for calibration of volumetric apparatus used in the laboratory.

• Scope:            

This SOP is applicable for calibration of volumetric apparatus in Quality Control.

• Responsibilities:

Chemist QC /Officer                       : To follow the procedure

• Accountability:

Head – Quality Control :  For System Compliance.

• Procedure:
  • Volumetric glassware shall be calibrated at 23°C to 27°C.
  • The volumetric apparatus shall be calibrated once in a year. For calibration select randomly two glassware of a particular volume capacity. Serial number on the glassware shall be considered the identification of the glassware during calibration. Only class A grade glass ware shall be used in quality control laboratory.
  • Clean the glassware for calibration to remove any grease, dirty etc. by keeping it immersed in chromic acid for 30 minutes. 
  • Rinse the glass ware under running tap water then rinse it with twice with water and dry it at 50°C for 120 minutes. Insure the glassware is completely dry.
  • Remove the glassware from the oven and keep it in dessictors, insure the ambient temperature is 25°C±2°C.
  • Arrange a water bath consisting of beaker, containing water.
  • Maintain the temperature of water bath at 25°C±0.5°C, throughout the calibration.
  • Place the apparatus to be calibrated on the balance and note the weight (A), remove the apparatus and transfer the water at 25°C. So that check the liquid in the apparatus mark label.
  • Weigh the apparatus and note down the weight (B).
  • Calculate the volume of glassware, using following formula.

               (B-A)

      =——————————

                   0.99602

           Where 0.99602 is the density of water at 25°C.

• Calibration of Volumetric flask (IS 915:1957):
• Maintain the temperature of the water bath at  25°C±0.5°C throughout the calibration.
• Tare the volumetric flask and fill the water up to mark and weigh. Calculate the volume of flask using the formula given below:                       

                               Weight of water (g)

            Volume =   ————————————-

                               Density of water

• Tolerance Limit: As given in annexure I.
  • Calibration of Burette (IS 1997:1967):
    • Maintain the temperature of the water bath at  25°C±0.5°C throughout the calibration.
    • Fill the burette with water up to the zero point.
    • Tare the beaker.
    • Empty the burette up to 10.0 ml in the suitable beaker and weigh.
    • Calculate the volume using the formula given below:

                                    Weight of water (g)

            Volume =    ————————————

                                 Density of water

• Similarly weigh the water up to 20.0, 30.0, 40.0 & 50.0 ml as applicable and calculate the volume using the above given formula and record.
  • Calibration of Bulb Pipette (IS 1117:1975):
    • Maintain the temperature of the water bath at  25°C±0.5°C throughout the calibration..
    • Tare a suitable beaker.
    • Pipette the water up to the mark and empty the pipette in tarred beaker and weigh.
    • Calculate the volume using the formula given below:

                             Weight of water (g)

           Volume =———————————–

                  Density of water

Calibration of Graduated Pipette (IS 4162:1967):

Maintain the temperature of the water bath at 25°C±0.5°C throughout the calibration.

Tare a suitable beaker.

Weigh the volume of water at different graduation in suitable tarred beaker and record.

Calculate the volume using the formula given below:

                                    Weight of water (g)

Volume =    ———————————–

                                       Density of water

Calibration of Measuring Cylinders (ISO4788:2005)

Maintain the temperature of the water bath at 25°C±0.5°C throughout the calibration.

Tare the measuring cylinder and fill the water up to mark and weigh.

Calculate the volume using the formula given below:

                                      Weight of water (g)

    Volume =    ———————————–

                                      Density of water

• • Broken glass wares shall not be used in the laboratory.
  • If any glassware breaks, then inform to Head Quality Control.
• Annexure(S):
• NA

• Reference (S) ,

IS 915:1957, IS 1997:1967, IS 1117:1975, IS 4162:1967, ISO4788:2005 

• Glossary           

SOP                :           Standard Operating Procedure

QA                   :           Quality Assurance

QC                  :           Quality Control

IS                     :           Indian Standard

ºC                    :           Degree Celsius

ml                    :           Milli Litre

ISO                  :           International Standard organization

• Revision History

Rev.  No.	Details of changes	Reason for change