| Sr. No. | Method Parameters | Observation |
| 1. | Column Discription | |
| 2. | Mobile Phase | |
| 3. | Column Length | |
| 4. | Particle Size | |
| 5. | Diameter | |
| 6. | Wavelength | |
| 7. | Flow Rate | |
| 8. | Injection volume | |
| 9. | Column Oven Temperature | |
| 10. | Preposed Method Regeneration |
CONCLUSION: Column Regeneration is satisfactory / not satisfactory
