by In epifluorescence microscopy, a parallel beam of light is passed directly upwards through the sample, maximizing the amount of illumination. This is also referred to as widefield microscopy. Like in any fluorescence microscope, a high-intensity light source is used.
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In epifluorescence microscopy, a parallel beam of light is passed directly upwards through the sample, maximizing the amount of illumination. This is also referred to as widefield microscopy. Like in any fluorescence microscope, a high-intensity light source is used. In epifluorescence microscopes, both the illuminated and emitted light travel through the same objective lens – hence the term “epi” from Greek, meaning “same”.
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A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. “Fluorescence microscope” refers to any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image.
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An upright fluorescence microscope (Olympus BX61) with the fluorescence filter cube turret above the objective lenses.
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Epifluorescence microscopy is particularly useful when imaging thick samples, over 10µm deep. However, the intense illumination and excitation of molecules outside the focal plane can produce images with a high background signal, when compared to other illumination modes such as TIRF and HILO.
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