Fluorescence microscopy

A fluorescence microscope is much the same as a conventional light microscope with added features to enhance its capabilities.  This fluorescent species in turn emits a lower energy light of a longer wavelength that produces the magnified image instead of the original light source.

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A fluorescence microscope is much the same as a conventional light microscope with added features to enhance its capabilities.

• The conventional microscope uses visible light (400-700 nanometers) to illuminate and produce a magnified image of a sample.
• A fluorescence microscope, on the other hand, uses a much higher intensity light source which excites a fluorescent species in a sample of interest. This fluorescent species in turn emits a lower energy light of a longer wavelength that produces the magnified image instead of the original light source.
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Fluorescent microscopy is often used to image specific features of small specimens such as microbes. It is also used to visually enhance 3-D features at small scales. This can be accomplished by attaching fluorescent tags to anti-bodies that in turn attach to targeted features, or by staining in a less specific manner. When the reflected light and background fluorescence is filtered in this type of microscopy the targeted parts of a given sample can be imaged. This gives an investigator the ability to visualize desired organelles or unique surface features of a sample of interest. This is achieved by using powerful light sources, such as lasers, that can be focused to a pinpoint. This focusing is done repeatedly throughout one level of a specimen after another. Most often an image reconstruction program pieces the multi level image data together into a 3-D reconstruction of the targeted sample.

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Fluorescent Microscope Image.

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