PROCEDURE FOR CLEANING OF LABORATORY GLASSWARE

1. OBJECTIVE:

To lay down a procedure for cleaning of laboratory glassware.

• SCOPE:

This procedure is applicable for procedure for cleaning of laboratory glassware.

• RESPONSIBILITY:

Microbiologist

• ACCOUNTABILITY:

QC Manager.

•  PROCEDURE:
  • Collect the used glassware after sterilization at 121⁰C & 15 lbs pressure for 30 min.
  • Remove the media for plates & test tubes.
  • Remove the label or glass marking by using cotton soaked isopropyl alcohol solution.
  • Clean the glassware thoroughly with water by using nylon brush if required.
  • Take about 1 lit. of savlon 2.5% or Dettol 2.5% (Use Disinfecatct alternative basis). Disinfectant quantity take more than 1.0 lit as per requirement.
  • Take disinfectant with carefully in glassware and stand for 10 – 15 minutes.
  • Again wash 2 to 3 times with water.
  • Finally 2 to 3 times rinse with purified water.
  • Keep the glassware in the oven at 180°C for drying about 30 minute.
  • After drying; remove the glassware from drying oven.
  • Put the Cleaned glassware where location has been defined for storage of cleaned glassware.
  • Ensure proper cleaning at the time of storage and if cleaning is not up to the mark then repeat the cleaning of glassware.
• TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the microbiology departments

• DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           Microbiology Department

Display Copy                 :           Microbiology Department (If Required)

• ATTACHMENTS:

• REFERENCES:

In-House

1. ABBREVIATIONS:

1. REVISION HISTORY OF CHANGE: