PROCEDURE FOR OPERATION & CALIBRATION OF pH METER

1.0 OBJECTIVE:

To lay down a procedure for Operation & Calibration of pH meter.

2.0 SCOPE:

This procedure is applicable for procedure for Operation & Calibration of pH meter .

3.0 RESPONSIBILITY:

Microbiologist

4.0 ACCOUNTABILITY:

QC Manager / Microbiology In charge

 5.0 PROCEDURE:

5.1 Operation Procedure:

• Ensure that the instrument is clean and suitable for use.
• Ensure the calibration of instrument is done.
• Switch ‘ON’ the mains.
• Put the electrode in sample. Set the temperature by knob about 25 centigrade and check the pH by pH/S/mV Knob.
• Display the pH in 0.00, note the pH.
• Maintain its usage log book .

5.2 Calibration Procedure:

• Ensure that the instrument is clean and suitable for use.
• Switch ‘ON’ the mains.
• Wash the pH electrode with purified water 2-3 times.
• Deep the pH electrode in buffer solution pH 4.00 and operated by Step no. 5.1.4 to 5.1.6.
• Check the pH of buffer solution pH 4.00; note down the pH in format of calibration of pH meter.
• After check the pH of buffer solution pH 4.00; remove the pH electrode and wash with purified water 2 – 3 times. And then deep the pH electrode in buffer solution pH 7.00 for observation.
• And after check the pH of buffer solution pH 7.00; remove the electrode and wash with purified water.
• Deep the electrode in buffer solution pH 9.20 and observed the pH.
• After completion of manual calibration check the pH 4.00, 7.00 & 9.20 one by one fill the Format .
• Prepared the ph buffer solution as per Format & affix the label as per Format .
• After use of tablet / capsule of pH buffer; note down in Format .

6.0 TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the microbiology departments

7.0 DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           Microbiology Department

Display Copy                 :           Microbiology Department (If Required)

8.0 ATTACHMENTS:

9.0 REFERENCES:

In-House

10.0 ABBREVIATIONS:

11.0 REVISION HISTORY OF CHANGE: