OPERATION AND CALIBRATION OF HOT AIR OVEN

This SOP describes the procedure for operation & calibration of hot air oven.

2.0 SCOPE:

This SOP is applicable to the procedure for operation & calibration of hot air oven .

3.0 RESPONSIBILITY:

             Microbiologist

4.0 ACCOUNTABILITY:

QC Manager.

 5.0 PROCEDURE:

5.1 Operation Procedure:

• Connect the 3 pin cord to main line and switch ‘ON’ the unit, and adjust the Temp.
• ‘On’ the red main button of instrument.
• ‘On’ the green load button of instrument.
• ‘On’ the yellow fan button of instrument.
• Speed adjusting Knob on Low, medium & High as per test requirements.
• Press the up & down arrow key; select the Set 1 and set the temperature to 180⁰C by press the both up arrow & set key. Hold the temperature about 180⁰C for 30 minute.
• Now the digital display indicator indicates the set temperature in green colour
• Actual temperature of hot air oven in red colour; display on instrument.
• Load no. of cycle is expressed in DSYYMMDD-00.
• DS is expressed in Dry Sterilization.
• YY is expressed in year.
• MM is expressed in month.
• DD is expressed in date.
• 00 is expressed no. of cycle done in same day.
• Maintain its usages log book .

5.2 Calibration:

• Set the temperature at 50, 60, 70, 80, 90, 100, 150 & 180ºC and record the results in Calibration Record .
• Limit of temperature variation is ± 2ºC from the desired temperature.
• Frequency of oven calibration is once in three months.

6.0 TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the microbiology departments

7.0 DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           Microbiology Department

Display Copy                 :           Microbiology Department (If Required)

8.0 ATTACHMENTS:

Sr. No.	Title
01.	Usage log book of Hot Air Oven
02.	Calibration Record of Hot Air Oven

9.0 REFERENCES:

In-House

10.0 ABBREVIATIONS:

Abbreviations	Extended Form
SOP	Standard Operating Procedure
NA	Not Applicable
MB	Microbiology
Sr. No.	Serial Number
QA	Quality Assurance
DEPT.	Department
IPA	Isopropyl Alcohol
%	Percentage

11.0 REVISION HISTORY OF CHANGE:

Sr. No.	Date	Revision Details	Revision No.
01	……..	NEW SOP	00