PROCEDURE FOR MICROBIAL CONTAMINATION TEST FOR COMPRESSED AIR

1. OBJECTIVE:

To lay down a procedure for microbial contamination test for compressed air.

• SCOPE:

This procedure is applicable for procedure for microbial contamination test for compressed air.

• RESPONSIBILITY:

Microbiologist

• ACCOUNTABILITY:

QC Manager.

•  PROCEDURE:
  • Take the sample by auto sampler, Note down the sampling details of compressed air.
  • Use the preincubated plate in the air sampler. Prepare the preincubated plate: 28G of nutrient agar mix with purified water to 100ml sterilize by steam sterilizer at 121⁰C, 15 lbs for 15 min.
  • Total Bacterial Contamination:
  • Pass the sample about 10 liter by air sampler.
  • Incubate the plate at 30-35⁰C for 5 days.
  • Acceptance criteria: should be absent /10 lit. sample
  • Total Fungal Contamination:
  • Pass the sample about 10 liter by air sampler.
  • Incubate the plate at 20-25⁰C for 5 days.
  • Acceptance criteria: should be absent /10 lit. sample
  • Maintain the record.

• TRAINING:

Trainer   : Manager – Quality Control

Trainees : Staff of the microbiology departments

• DISTRIBUTION:

Master Copy                  :           QA Department

Controlled Copy            :           Microbiology Department

Display Copy                 :           Microbiology Department (If Required)

• REFERENCES:

In-House

1. ABBREVIATIONS:

Abbreviations	Extended Form
SOP	Standard Operating Procedure
NA	Not Applicable
MB	Microbiology
Sr. No.	Serial Number
QA	Quality Assurance
DEPT.	Department
IPA	Isopropyl Alcohol
%	Percentage