by 1.0 OBJECTIVE:
To lay down a procedure for Operation & Calibration of pH meter.
2.0 SCOPE:
This procedure is applicable for procedure for Operation & Calibration of pH meter .
3.0 RESPONSIBILITY:
Microbiologist
4.0 ACCOUNTABILITY:
QC Manager / Microbiology In charge
5.0 PROCEDURE:
5.1 Operation Procedure:
- Ensure that the instrument is clean and suitable for use.
- Ensure the calibration of instrument is done.
- Switch ‘ON’ the mains.
- Put the electrode in sample. Set the temperature by knob about 25 centigrade and check the pH by pH/S/mV Knob.
- Display the pH in 0.00, note the pH.
- Maintain its usage log book .
5.2 Calibration Procedure:
- Ensure that the instrument is clean and suitable for use.
- Switch ‘ON’ the mains.
- Wash the pH electrode with purified water 2-3 times.
- Deep the pH electrode in buffer solution pH 4.00 and operated by Step no. 5.1.4 to 5.1.6.
- Check the pH of buffer solution pH 4.00; note down the pH in format of calibration of pH meter.
- After check the pH of buffer solution pH 4.00; remove the pH electrode and wash with purified water 2 – 3 times. And then deep the pH electrode in buffer solution pH 7.00 for observation.
- And after check the pH of buffer solution pH 7.00; remove the electrode and wash with purified water.
- Deep the electrode in buffer solution pH 9.20 and observed the pH.
- After completion of manual calibration check the pH 4.00, 7.00 & 9.20 one by one fill the Format .
- Prepared the ph buffer solution as per Format & affix the label as per Format .
- After use of tablet / capsule of pH buffer; note down in Format .
6.0 TRAINING:
Trainer : Manager – Quality Control
Trainees : Staff of the microbiology departments
7.0 DISTRIBUTION:
Master Copy : QA Department
Controlled Copy : Microbiology Department
Display Copy : Microbiology Department (If Required)
8.0 ATTACHMENTS:
| Sr. No. | Title |
| 01. | Usage Record of pH Meter |
| 02. | Calibration Record of pH |
| 03. | pH Buffer Preparation Record |
| 04. | Label for pH Buffer |
| 05. | Consumption Record of pH Buffer Tablets / Capsules |
9.0 REFERENCES:
In-House
10.0 ABBREVIATIONS:
| Abbreviations | Extended Form |
| SOP | Standard Operating Procedure |
| NA | Not Applicable |
| MB | Microbiology |
| Sr. No. | Serial Number |
| QA | Quality Assurance |
| DEPT. | Department |
11.0 REVISION HISTORY OF CHANGE:
| Sr. No. | Date | Revision Details | Revision No. |
| 01 | ……….. | NEW SOP | 00 |
