- OBJECTIVE:
To lay down a procedure for Pipetting.
- SCOPE:
This procedure is applicable for procedure for Pipetting.
- RESPONSIBILITY:
Microbiologist
- ACCOUNTABILITY:
QC Manager.
- PROCEDURE:
- Ensure that the Pipette is cleaned and dried before use.
- Before any dilution, rinse the pipette with same solution, which is to be used for dilution.
- Fill the pipette with same solution with which dilution is to be made and adjust zero labels.
- Make dilution as per requirement but do not use same pipette in next dilution. Use separate pipette for lower dilution.
- Make dilution slowly by adding solutions from pipette and also ensure that at the tip of pipette, solution is not remaining.
- After dilution keep pipette in washing area for washing and drying purpose.
- TRAINING:
Trainer : Manager – Quality Control
Trainees : Staff of the microbiology departments
- DISTRIBUTION:
Master Copy : QA Department
Controlled Copy : Microbiology Department
Display Copy : Microbiology Department (If Required)
- ATTACHMENTS:
Sr. No. | Title | Annexure No. |
01. | NA | NA |
- REFERENCES:
In-House
- ABBREVIATIONS:
Abbreviations | Extended Form |
SOP | Standard Operating Procedure |
NA | Not Applicable |
MB | Microbiology |
Sr. No. | Serial Number |
QA | Quality Assurance |
DEPT. | Department |
IPA | Isopropyl Alcohol |
% | Percentage |