Procedure to Work Under Fume Hood

1. Objective

            To lay down the procedure for working under fume hood.

•  Scope

 This SOP is applicable in Quality Control during working under fumigation hood. It is  used for handling and usage of inflammable solvents, acids and highly volatile solvents.

•  Responsibility

           Chemist QC    

• Accountability

HOD QC

• Procedure
  • Cleaning
    • Rinse all the glass ware immediately after use with water and send for washing.
    • Clean the sink with water.
    • Clean the platform with water.
    • Mop the hood with clean cloth.
  • Preliminary Checks
    • Check the hood for proper functioning and switches for their functioning.
    • Check the blower for proper functioning.
    • Availability of safety dress .i.e. Acid resistant apron, chemical mask, rubber hand gloves and goggles / safety face mask.
    • Availability of all reagents / documents / protocols for performing the test.
    • Availability of fire safety i.e. sand bucket and fire extinguisher.
  • Working
    • Enter in the hot zone.
    • Adjust the sash height as required

• Wear the safety dress i.e. Acid resistant apron, rubber hand gloves and goggles. If required use chemical mask / safety face mask.
  • Switch on the tube light marked as tube light.
  • Push the green button of the starter, placed at the wall left to hood, to ON the blower.
  • Use heating mantle to heat the solution. Do not use hot plate. 
  • Work as per approved protocols.
  • Drain the waste solvents in the sink. Do not drain the acids while hot.
  • Rinse all the glass ware with water and send for cleaning.
  • Clean the sink and plat form.
  • After completion of work, switch off the light and electrical heating if any.
  • Switch OFF the blower, by pushing the red button of starter.
  • Lower the slash completely after completion of work.
  •   Important:
    • Do not send the glass wares as such for washing.
    • Do not switch off the blower while the sash is close.
    • If the acid spoiled, first neutralize with base and then wash with water current.
    • In case of solvent, spread the sand on the solvent. Clean and send to the ETP.
    • In case of any incident immediately take first aid and inform to HOD QC. 
•       Annexure (S)

  NA

•       Reference (S)

              In House

• Glossary

QC	:	Quality control
SOP	:	Standard Operating Procedure
HOD	:	Head of Department

• Revision History

Rev. No.	Details of changes	Reason for change